Definition of supernatant in English:



  • Denoting the liquid lying above a solid residue after crystallization, precipitation, centrifugation, or other process.

    • ‘Samples were then centrifuged (17,000 × g for 10 min), and supernatant fluid was stored at - 20°C for VFA analysis.’
    • ‘Ammonia N was determined on the supernatant fluid using the phenol-hypochlorite procedure.’
    • ‘The supernatant fluid was filtered and centrifuged.’
    • ‘The resultant supernatant fraction was used for the determination of lipid peroxidation and glutathione.’


  • A volume of supernatant liquid.

    • ‘The tubes were centrifuged at 5000 rpm for 2 min, and the supernatant discarded.’
    • ‘After various time intervals the supernatants were removed, cell samples were fixed with paraformaldehyde solution for 30 minutes at room temperature.’
    • ‘All extracts were centrifuged at 10000 rpm for 5 min, and the supernatants were then evaporated under vacuum to dryness and resuspended in appropriate solvents to a final concentration of 1 mg/ml.’
    • ‘The homogenates were then centrifuged at 3000 rpm for 15 min and the supernatants were used for determination of the lipid peroxide level via the MDA method, as described above.’
    • ‘The suspensions were spun at 1000 g for 30 minutes and the supernatants were filtered and lyophilized.’