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verb[WITH OBJECT]usually be sonicated
Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes.
- ‘Samples were sonicated for 5 sec four times to shear genomic DNA and reduce viscosity.’
- ‘The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter.’
- ‘After dialysis the residue was sonicated and ready for use.’
- ‘The sample was sonicated to clarity in a sonication bath (Ultrawave).’
- ‘The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above.’
A biological sample that has been sonicated.
- ‘The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.’
- ‘Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.’
- ‘Reactions were initiated by the addition of crude cell sonicates to prewarmed solutions.’
- ‘The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).’
- ‘The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected.’
1950s: from sonic + -ate.
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