Definition of trypan blue in English:

trypan blue


mass noun
  • A diazo dye used as a biological stain due to its absorption by macrophages of the reticuloendothelial system.

    • ‘Low levels of positively stained trypan blue dye were demonstrated in all subject groups, consistent with high viability.’
    • ‘The cells were released for implantation if the cell viability was more than 85%, as determined with trypan blue staining.’
    • ‘BAL cells were stained with trypan blue, and viable cells were counted using a hemocytometer.’
    • ‘At the end of ultrasound exposure, cells were stained with trypan blue.’
    • ‘Differential cell counts and trypan blue staining revealed a purity and viability of isolated macrophages of greater than 95% in all groups.’


Early 20th century: trypan from trypanosome.