Definition of transfect in English:



[WITH OBJECT]Microbiology
  • 1 Infect (a cell) with free nucleic acid.

    ‘cells were transfected by electroporation’
    • ‘Schneider cells were seeded onto cover slips and after 24 hr the cells were transfected with plasmid pBSHS-Fl d.’
    • ‘MC57 cells were also transfected by electroporation and selected in 1.2 mg/ml G418.’
    • ‘Stably transfected cells were selected with 500 g/ml G418 (Sigma).’
    • ‘Cells were transfected with eYFP-C10HRas 3-4 days before the experiment.’
    • ‘Cells were grown to a density of 5 x 10 cells/ml and transfected by electroporation.’
    1. 1.1Introduce (genetic material) by infecting a cell with free nucleic acid.
      • ‘An increase was seen in all 7 rabbits transfected with alkaline phosphatase plasmid.’
      • ‘PUVA-damaged and undamaged DNA was transfected into a yeast strain containing the ADE2 gene regulated by a p53-responsive promoter.’
      • ‘I have purified, cloned, sub-cloned, transfected, blotted and detected DNA.’
      • ‘It was known that there were certain ‘poison sequences’ which might exist upstream of the encoding area of the gene, which would be desirable to remove before transfecting the gene into COS cells.’
      • ‘HEK293 cells were cultured in 100 × 20 mm regular culture dishes and transfected with 5 g DNA.’


1960s: from trans- ‘across’+ infect, or a blend of transfer and infect.