Definition of transfect in English:

transfect

verb

[WITH OBJECT]Microbiology
  • 1 Infect (a cell) with free nucleic acid:

    ‘cells were transfected by electroporation’
    • ‘Cells were grown to a density of 5 x 10 cells/ml and transfected by electroporation.’
    • ‘MC57 cells were also transfected by electroporation and selected in 1.2 mg/ml G418.’
    • ‘Stably transfected cells were selected with 500 g/ml G418 (Sigma).’
    • ‘Schneider cells were seeded onto cover slips and after 24 hr the cells were transfected with plasmid pBSHS-Fl d.’
    • ‘Cells were transfected with eYFP-C10HRas 3-4 days before the experiment.’
    1. 1.1 Introduce (genetic material) by infecting a cell with free nucleic acid.
      • ‘It was known that there were certain ‘poison sequences’ which might exist upstream of the encoding area of the gene, which would be desirable to remove before transfecting the gene into COS cells.’
      • ‘PUVA-damaged and undamaged DNA was transfected into a yeast strain containing the ADE2 gene regulated by a p53-responsive promoter.’
      • ‘I have purified, cloned, sub-cloned, transfected, blotted and detected DNA.’
      • ‘HEK293 cells were cultured in 100 × 20 mm regular culture dishes and transfected with 5 g DNA.’
      • ‘An increase was seen in all 7 rabbits transfected with alkaline phosphatase plasmid.’

Origin

1960s: from trans- ‘across’+ infect, or a blend of transfer and infect.

Pronunciation:

transfect

/tranzˈfɛkt/