Definition of immunoblotting in English:



mass nounBiochemistry
  • A technique for analysing or identifying proteins in a mixture, involving separation by electrophoresis followed by staining with antibodies.

    • ‘The samples were processed by immunoprecipitation followed by immunoblotting.’
    • ‘Cells were harvested and protein extracts were prepared for immunoprecipitation and immunoblotting with the indicated antibodies.’
    • ‘Extraction of total soluble protein from tobacco leaves and immunoblotting using the above antibody was carried out according to standard protocols.’
    • ‘The specificity of this antibody towards wood peroxidases was confirmed by immunoblotting.’
    • ‘Fraction purities were assessed by a combination of Coomassie staining and immunoblotting of electrophoretically separated proteins.’